Learning to Detect and Track Cells for Quantitative Analysis of Time-Lapse Microscopic Image Sequences

© 2015 IEEE.Studying the behaviour of cells using time-lapse microscopic imaging requires automated processing pipelines that enable quantitative analysis of a large number of cells. We propose a pipeline based on state-of-the-art methods for background motion compensation, cell detection, and tracking which are integrated into a novel semi-automated, learning based analysis tool. Motion compensation is performed by employing an efficient nonlinear registration method based on powerful discrete graph optimisation. Robust detection and tracking of cells is based on classifier learning which only requires a small number of manual annotations. Cell motion trajectories are generated using a recent global data association method and linear programming. Our approach is robust to the presence of significant motion and imaging artifacts. Promising results are presented on different sets of in-vivo fluorescent microscopic image sequences

Surface emitting lasers with combined output

Surface emitting lasers are laterally aligned and coupled together and also have their light output signals combined. This results in greater phase and frequency coherency and narrower and reduced amplitude sidelobes. Preferably, not more than two lasers are longitudinally aligned along the same axis for still greater coherency compared with adding the light output signals of more than two longitudinally aligned lasers. The lasers can be of the DH-LOC type or of the QW type

Clearing sandplain country

THE greatest scope for development of new land in Western Australia lies in the millions of acres of sandplain country along the west and south coasts and to a lesser extent in the Newdegate area. With the development, or at least alienation, of the South-West and the wheatbelt nearing completion, attention has been focused on these tracts of sandplain country

Light land development in the West Midlands

Although it has not received the widespread publicity which has attended land settlement schemes on the south coastal plain some very successful light land development has taken place in the West Midlands during the past 10 years. Large areas of what was once unattractive scrub country are now carrying highly productive pastures and the progress made by Mr. J. W. White at Badgingarra is an example of what may be achieved in this district by using proven techniques

Barrel Medic for wheatbelt pastures - Demonstrations show promise

Investigations with Barrel Medic (Medicago tribuloides) have indicated that this has a wide range of usefulness as a leguminous pastoral plant for the heavier soils those areas receiving less than 18 inches of rainfall annually There has been a continued emphasis on improved pasture as the basis for better and more productive rotations and the outstanding part played by subterranean clover in utilising large areas of light sandplain in the higher rainfall districts is well known. Barrel medic (or Barrel Clover as it is sometimes called) should fill a somewhat similar position under the conditions for which it is suited

Linear laser diode arrays for improvement in optical disk recording for space stations

The design and fabrication of individually addressable laser diode arrays for high performance magneto-optic recording systems are presented. Ten diode arrays with 30 mW cW light output, linear light vs. current characteristics and single longitudinal mode spectrum were fabricated using channel substrate planar (CSP) structures. Preliminary results on the inverse CSP structure, whose fabrication is less critically dependent on device parameters than the CSP, are also presented. The impact of systems parameters and requirements, in particular, the effect of feedback on laser design is assessed, and techniques to reduce feedback or minimize its effect on systems performance, including mode-stabilized structures, are evaluated

Stocking rate and pasture management

FARMERS west of the Great Southern Railway, raising sheep and beef cattle, frequently associate soil deficiencies with difficulties in holding sub. clover in their pastures. Field observations and some trials in those districts indicate that in many paddocks the problem arises through undergrazing rather than a deficiency in the soil

Quantum discord in spin-cluster materials

The total quantum correlation (discord) in Heisenberg dimers is expressed via the spin-spin correlation function, internal energy, specific heat or magnetic susceptibility. This allows one to indirectly measure the discord through neutron scattering, as well as calorimetric or magnetometric experiments. Using the available experimental data, we found the discord for a number of binuclear Heisenberg substances with both antiferro- and ferromagnetic interactions. For the dimerized antiferromagnet copper nitrate Cu(NO_3)_2*2.5H_2O, the three independent experimental methods named above lead to a discord of approximately 0.2-0.3 bit/dimer at a temperature of 4 K. We also determined the temperature behavior of discord for hydrated and anhydrous copper acetates, as well as for the ferromagnetic binuclear copper acetate complex [Cu_2L(OAc)]*6H_2O, where L is a ligand.Comment: 7 pages, 6 figure

AlGaAs heterojunction lasers

The characterization of 8300 A lasers was broadened, especially in the area of beam quality. Modulation rates up to 2 Gbit/sec at output powers of 20 mW were observed, waveform fidelity was fully adequate for low BER data transmission, and wavefront measurements showed that phase aberrations were less than lamda/50. Also, individually addressable arrays of up to ten contiguous diode lasers were fabricated and tested. Each laser operates at powers up to 30 mW CW in single spatial mode. Shifting the operating wavelength of the basic CSP laser from 8300 A to 8650 A was accomplished by the addition of Si to the active region. Output power has reached 100 mW single mode, with excellent far field wave front properties. Operating life is currently approx. 1000 hrs at 35 mW CW. In addition, laser reliability, for operation at both 8300 A and 8650 A, has profited significantly from several developments in the processing procedures